In vivo efficacy of tobramycin-loaded synthetic calcium phosphate beads in a rabbit model of staphylococcal osteomyelitis
In vivo efficacy of tobramycin-loaded synthetic calcium phosphate beads in a rabbit model of staphylococcal osteomyelitis
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Abstract Background Osteomyelitis is an acute or chronic inflammatory process of the bone following infection with pyogenic organisms like Staphylococcus aureus.Tobramycin (TOB) is a promising aminoglycoside antibiotic used to treat various bacterial infections, including S.aureus.The aim of this study was to investigate the efficacy of tobramycin-loaded calcium phosphate beads (CPB) in a rabbit osteomyelitis model.
Methods Tobramycin (30 mg/mL) was incorporated into CPB by dipping method and the efficacy of TOB-loaded CPB was studied in a rabbit osteomyelitis model.For juxtaposition, CPB with and without TOB were prepared.Twenty-five New Zealand white rabbits were grouped (n = 5) as sham (group 1), TOB-loaded CPB without S.aureus (group 2), S.
aureus only (group 3), S.aureus + CPB (group 4), and S.aureus + TOB-loaded CPB (group 5).Groups infected with S.
aureus followed by CPB implantation were immediately subjected click here to surgery at the mid-shaft of the tibia.After 28 days post-surgery, all rabbits were euthanized and the presence or absence of chronic osteomyelitis and the extent of architectural destruction of the bone were assessed by radiology, bacteriology and histological studies.Results Tobramycin-loaded CPB group potentially inhibited the growth of S.aureus causing 3.
2 to 3.4 log10 reductions in CFU/g of bone tissue compared to the controls.Untreated groups infected with S.aureus showed signs of chronic osteomyelitis with abundant bacterial growth and alterations in bone architecture.
The sham group and TOB-loaded CPB group showed no evidence of bacterial growth.Conclusions here TOB-incorporated into CPB for local bone administration was proven to be more successful in increasing the efficacy of TOB in this rabbit osteomyelitis model and hence could represent a good alternative to other formulations used in the treatment of osteomyelitis.